Essa Dirandeh; Mohammad Kazemi Fard; Tannaz Saberifar
Volume 24, Issue 3 , October 2022, , Pages 373-382
Abstract
To investigate the effect of fatty liver on insulin resistance in the liver of laying hens, an experiment using 80 laying hens of commercial line strains (w-36) after peak production (age 43 weeks) for eight weeks were performedin a completely randomized design with two treatments. The experimental treatments ...
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To investigate the effect of fatty liver on insulin resistance in the liver of laying hens, an experiment using 80 laying hens of commercial line strains (w-36) after peak production (age 43 weeks) for eight weeks were performedin a completely randomized design with two treatments. The experimental treatments included control group (no injection) and the estradiol group (injection of two mg estradiol benzoate per kg body weight). In order to induce fatty liver disease, the injection of 17-beta estradiol started from the third week of experiment (age 46), and was performed three times a week for 21 days. Blood samples were taken to evaluate the concentration of triglycerides, cholesterol and liver enzymes (aspartate transaminase (AST), alanin transaminase (ALT) and alkaline phosphatase (ALP) at the end of the experiment using 20 hens from each treatment. At the end of experiment, five hens of each treatment were selected and sacrificed, then 50 g of liver tissue was removed to study gene expression of insulin receptor (InR), glucose transporter1 (Glut1), sterol regulatory element binding protein (SREBP1), Ribosomal S6 kinase1 (S6K1), Target of Rapamycin (TOR) and Forkhead box protein O1 (FOXO1). The results showed that the injection of estradiol induced fatty liver and increased plasma concentrations of cholesterol and triglyceride as well as activity of AST, ALT and ALP. In hens with fatty liver, expression of FOXO1 (4.1-fold), TOR (3.9-fold), S6K1 (3.3-fold) genes increased, and conversely, expression of InR (4.6-fold), Glut1 (7.5-fold) decreased. In conclusion results of the present study showed that the fatty liver induction in laying hens increased expression of insulin resistance-related genes.
Essa Dirandeh; Mohammad Kazemi Fard; Tannaz Saberifar
Volume 24, Issue 2 , July 2022, , Pages 227-235
Abstract
The occurrence and spread of inflammation can affect the quantity and quality of eggs in laying hens. Therefore, the aim of this study was to evaluate the effect of soy Gensitein (GEN)on systemic inflammatory status and performances of laying hens post-peak. The research was performed in a completely ...
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The occurrence and spread of inflammation can affect the quantity and quality of eggs in laying hens. Therefore, the aim of this study was to evaluate the effect of soy Gensitein (GEN)on systemic inflammatory status and performances of laying hens post-peak. The research was performed in a completely randomized design, during a period of about 8 weeks, using 80 laying hens of High Line W-36 strains (older than 43 weeks). Experimental treatments were control group (basal diet) and genistein group (basal diet+ 20mg GEN/kg of diet). At the end of experiment, five hens from each treatment were laughter and 50 g of liver sample were taken for gene expression of IL-1, IL-2, TNF-α, COX-1 and COX-2. Results showed that soy GEN decreased gene expression of IL-1 (245 fold), IL-2 (3.53 fold), IL-6 (2.68 fold), TNF-α (4.83 fold), COX-1 (3.92 fold) and COX-2 (1.73 fold) compared to the control group. Laying hens fed GEN diets had higher feed intake and better conversion ratio and had higher egg production rates than the control group.The results of this study showed that soy GEN could reduce inflammation in the liver of laying hens post-peak and improve production performances.
Seyed Hamed Mousavi Alamdardehi; Zarbakht Ansari Pirsaraei; Essa Dirandeh; Mohammad Kazemi Fard
Volume 23, Issue 4 , January 2022, , Pages 525-533
Abstract
Effect of Basil powder and extract on yield and carcass characteristics, relative expression of heat shock protein gene (HSP70) and some blood parameters of broiler chickens under heat stress using 160 Ross 308 one-day-old broiler chicks in a completely randomized design with 5 treatments and 4 replications ...
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Effect of Basil powder and extract on yield and carcass characteristics, relative expression of heat shock protein gene (HSP70) and some blood parameters of broiler chickens under heat stress using 160 Ross 308 one-day-old broiler chicks in a completely randomized design with 5 treatments and 4 replications (8 chickens per replication), was evaluated for 42 days. Treatments included: 1- Basic diet (control), 2- Basic diet+ Vitamin C (250 mg per kg of grain), 3- Basic diet + 25 mg of Basil powder (per kg of grain), 4- Basic diet +50 mg of Basil powder (per kg of grain), 5- The use of Basil extract (1 ml/liter water). Feed intake, body weight gain, feed conversion ratio, carcass characteristics, blood parameters and relative expression of HSP70 gene in blood were measured. The effects of treatments on blood parameters and carcass characteristics were not significant. Relative expression of the HSP70 gene in the blood was significantly reduced in all treatments compared to the control (P<0.05). Based on the results, basil powder and extract reduce the negative effects of heat stress in broilers, so their use in broiler diets is recommended to prevent heat stress.
Essa Dirandeh
Volume 18, Issue 3 , October 2016, , Pages 575-582
Abstract
The aim of this study was to compare the efficiency of five synchronization protocols, economically, in Holstein cows during heat stress. This experiment was conducted at a commercial dairy farm in summer 2015 (temperature humidity index 73–81). Five hundred Holstein dairy cows with ˃25 kg/d milk ...
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The aim of this study was to compare the efficiency of five synchronization protocols, economically, in Holstein cows during heat stress. This experiment was conducted at a commercial dairy farm in summer 2015 (temperature humidity index 73–81). Five hundred Holstein dairy cows with ˃25 kg/d milk yield were randomly assigned to five experimental groups: 1) ovsynch; 2) presynch-ovsynch; 3) double ovsynch; 4) G7G-ovsynch and 5) PG7G-ovsynch. Results showed that synchronization rate was significantly lower in ovsynch compared to other protocols (P ˂ 0.05). Presynchronization of cows before initiation of ovsynch improved conception rates (CR) at 32 and 62 days (d) after AI compared to ovsynch protocol (P ˂ 0.05). According to the reproductive CR at 32 and 62 d after AI and pregnancy loss, it was found that G7G and PG7G were the best protocols compared to other protocols. The cost associated with the semen use per conception was highest at ovsynch and lowest at both G7G and PG7G protocols. Total costs per conception at 60 d after AI were lowest (1890000 rials) in G7G-ovsynch compared to other protocols. In conclusion, considering total costs associated with hormones and semen use per pregnancy, the G7G protocol is economically the most effective among the studied protocols.
Marjan Esmaili; Zarbakht Ansari Pirsaraie
Volume 18, Issue 3 , October 2016, , Pages 603-613
Abstract
The aim of this study was to investigate the effect of different concentration of royal jelly’s fatty acids (0.0, 125, 250, 500, 1000 µg/ml and 50 µl DMSO) on in vitro maturation of goat oocyte. Goat ovaries were collected from local slaughterhouse and transported to the laboratory ...
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The aim of this study was to investigate the effect of different concentration of royal jelly’s fatty acids (0.0, 125, 250, 500, 1000 µg/ml and 50 µl DMSO) on in vitro maturation of goat oocyte. Goat ovaries were collected from local slaughterhouse and transported to the laboratory and COCs were cultured into the maturation media supplemented with different concentration of royal jelly’s fatty acids. The results indicated that the increase of royal jelly’s fatty acids concentration from control to 250µg/mL were significantly (P